Journal article
RNA sequencing identifies novel translational biomarkers of kidney fibrosis
FL Craciun, V Bijol, AK Ajay, P Rao, RK Kumar, J Hutchinson, O Hofmann, N Joshi, JP Luyendyk, U Kusebauch, CL Moss, A Srivastava, J Himmelfarb, SS Waikar, RL Moritz, VS Vaidya
Journal of the American Society of Nephrology | AMER SOC NEPHROLOGY | Published : 2016
Abstract
CKD is the gradual, asymptomatic loss of kidney function, but current tests only identify CKD when significant loss has already happened. Several potential biomarkers of CKD have been reported, but none have been approved for preclinical or clinical use. Using RNA sequencing in a mouse model of folic acidinduced nephropathy, we identified ten genes that track kidney fibrosis development, the common pathologic finding in patients with CKD. The gene expression of all ten candidates was confirmed to be significantly higher (approximately ten- to 150-fold) in three well established, mechanistically distinct mouse models of kidney fibrosis than in models of nonfibrotic AKI. Protein expression of ..
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Awarded by National Institutes of Health
Funding Acknowledgements
[ "J.P.L. was supported by the National Institutes of Health (NIH)/National Institute of Environmental Health Sciences (R01-ES017537). R.L.M. was funded by the American Recovery and Reinvestment Act funds through NIH/National Human Genome Research Institute (RC2-HG005805), National Institute of General Medical Sciences (2P50 GM076547), and Center for Systems Biology (S10RR027584 and GM087221).", "The Boston Kidney Biopsy Cohort led by S.S.W. is supported by NIH/National Institute of Diabetes and Digestive and Kidney Diseases (DK093574). Resources were made available from NIH grant UH3TR000504 (to J.H.). Work in the Vaidya laboratory was supported by an Outstanding New Environmental Scientist award from NIH/National Institute of Environmental Health Sciences (ES017543) and an Innovation in Regulatory Science Award from Burroughs Wellcome Fund (BWF-1012518)." ]