Journal article

CRISPR/Cas9: A tool for immunological research

Katharina Hochheiser, Andrew J Kueh, Thomas Gebhardt, Marco J Herold

European Journal of Immunology | WILEY | Published : 2018

Abstract

The CRISPR/Cas9-system was originally identified as part of the adaptive immune system in bacteria and has since been adapted for the genetic manipulation of eukaryotic cells. The technique is of particular value for biomedical sciences, as it enables the genetic manipulation of cell lines and primary cells as well as whole organisms with unprecedented ease and efficiency. Furthermore, the CRISPR/Cas9-technology has the potential for future therapeutic applications in the clinic. Here, we discuss the use of CRISPR/Cas9 for the genetic modification of haematopoietic cells and the generation of mouse models for immunological research. Additionally, we explain how the technique can be applied a..

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Grants

Awarded by National Health and Medical Research Council, Australia


Funding Acknowledgements

We thank all of our current and past colleagues at The Walter and Eliza Hall Institute and elsewhere. Our work was supported by the German Research Foundation (to KH), the National Health and Medical Research Council, Australia (APP1145728, APP1143105), Venture Grant Cancer Council Victoria, Australian Phenomics Network (to MJH) and the Australian Research Council (to T.G.). T.G. is supported by a Senior Medical Research Fellowship from the Sylvia and Charles Viertel Charitable Foundation. This work was made possible through Victorian State Government Operational Infrastructure Support and Australian Government National Health and Medical Research Council Independent Research Institutes Infrastructure Support Scheme.