Circulating TFH cells, serological memory, and tissue compartmentalization shape human influenza-specific B cell immunity

M Koutsakos; AK Wheatley; L Loh; EB Clemens; S Sant; S Nüssing; A Fox; AW Chung; KL Laurie; AC Hurt; S Rockman; M Lappas; T Loudovaris; SI Mannering; GP Westall; M Elliot; SG Tangye; LM Wakim; SJ Kent; THO Nguyen; K Kedzierska

Journal article

Circulating TFH cells, serological memory, and tissue compartmentalization shape human influenza-specific B cell immunity

M Koutsakos, AK Wheatley, L Loh, EB Clemens, S Sant, S Nüssing, A Fox, AW Chung, KL Laurie, AC Hurt, S Rockman, M Lappas, T Loudovaris, SI Mannering, GP Westall, M Elliot, SG Tangye, LM Wakim, SJ Kent, THO Nguyen Show all

Science Translational Medicine | AMER ASSOC ADVANCEMENT SCIENCE | Published : 2018

DOI: 10.1126/scitranslmed.aan8405

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Abstract

Immunization with the inactivated influenza vaccine (IIV) remains the most effective strategy to combat seasonal influenza infections. IIV activates B cells and T follicular helper (TFH) cells and thus engenders antibody-secreting cells and serum antibody titers. However, the cellular events preceding generation of protective immunity in humans are inadequately understood. We undertook an in-depth analysis of B cell and T cell immune responses to IIV in 35 healthy adults. Using recombinant hemagglutinin (rHA) probes to dissect the quantity, phenotype, and isotype of influenza-specific B cells against A/California09-H1N1, A/Switzerland-H3N2, and B/Phuket, we showed that vaccination induced a ..

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University of Melbourne Researchers

Marios Koutsakos Author

Adam Wheatley Author

Liyen Loh Author

Bridie Clemens Author

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Grants

Awarded by National Health and Medical Research Council

Funding Acknowledgements

This work was supported by NHMRC program grants ID1071916 (K.K.) and ID1052979 (S.J.K.) and NHMRC project grants ID1129099 (A.K.W.) and ID1125164 (A.W.C.). M.K. and S.N. are supported by a Melbourne International Research Scholarship and Melbourne International Fee Remission Scholarship (MIFRS). E.B.C. is an NHMRC Peter Doherty Fellow. S.S. is supported by the Victoria-India Doctoral Scholarship and MIFRS. World Health Organization (WHO) Collaborating Centre for Reference Research on Influenza is supported by the Australian Government Department of Health. S.I.M. is supported by Juvenile Diabetes Research Foundation (JDRF) Career Development Award (5-CDA-2014-210-A-N) and NHMRC (ID1123586). M.L. is supported by a Career Development Fellowship from NHMRC (ID1047025). S.R. is an employee of Seqirus Ltd. S.G.T. is supported by NHMRC program and project grants. S.G.T., S.J.K., and K.K. are NHMRC Research Fellows.