Journal article

AN INTEGRATION-DEFECTIVE U5 DELETION MUTANT OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 REVERTS BY ELIMINATING ADDITIONAL LONG TERMINAL REPEAT SEQUENCES

E VICENZI, DS DIMITROV, A ENGELMAN, TS MIGONE, DFJ PURCELL, J LEONARD, G ENGLUND, MA MARTIN

JOURNAL OF VIROLOGY | AMER SOC MICROBIOLOGY | Published : 1994

Abstract

Nonoverlapping deletions that eliminated the 5' (HIV-1US/603del), middle (HIV-1U5/206del), and 3' (HIV-1U5/604del) thirds of the U5 region of the human immunodeficiency virus type 1 (HIV-1) long terminal repeat (LTR) were studied for their effects on virus replication (transient transfection of HeLa cells) and infectivity (T-cell lines and peripheral blood mononuclear cells). All three mutants exhibited a wild-type phenotype in directing the production and release of virus particles from transfected HeLa cells. In infectivity assays, HIV-1U5/206del was usually indistinguishable from wild-type virus whereas HIV-1U%/603del was unable to infect human peripheral blood mononuclear cells or MT4 an..

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University of Melbourne Researchers