Journal article

Sequential Membrane Rupture and Vesiculation during Plasmodium berghei Gametocyte Egress from the Red Blood Cell

Maria Andreadaki, Eric Hanssen, Elena Deligianni, Cyrille Claudet, Kai Wengelnik, Vanessa Mollard, Geoffrey I McFadden, Manouk Abkarian, Catherine Braun-Breton, Inga Siden-Kiamos

Scientific Reports | NATURE PUBLISHING GROUP | Published : 2018

Abstract

Malaria parasites alternate between intracellular and extracellular stages and successful egress from the host cell is crucial for continuation of the life cycle. We investigated egress of Plasmodium berghei gametocytes, an essential process taking place within a few minutes after uptake of a blood meal by the mosquito. Egress entails the rupture of two membranes surrounding the parasite: the parasitophorous vacuole membrane (PVM), and the red blood cell membrane (RBCM). High-speed video microscopy of 56 events revealed that egress in both genders comprises four well-defined phases, although each event is slightly different. The first phase is swelling of the host cell, followed by rupture a..

View full abstract

Grants

Awarded by General Secretariat for Research and Technology, Ministry of Education, Greece


Funding Acknowledgements

We thank Simon Conway for essential help with the scanning EM and Theodossia Bitzou for artwork. We are grateful to Robyn Webb and Richard Webb, of the Australian Microscopy & Microanalysis Research Facility at the Center for Microscopy and Microanalysis at the University of Queensland, for access to the 3View. Support was provided by a grant from the program Bilateral Research and Technology Cooperation Greece-France, The General Secretariat for Research and Technology, Ministry of Education, Greece (grant number 2013SE01380004). The post-doctoral research of MA was supported by a fellowship from IKY (State Scholarship Foundation) funded by the action "Support of Post-Doctoral Researchers" from the Operational Program "Development of Human Resources, Education and Life-Long Learning" with priority areas 6, 8, 9 and co-funded by the European Social Fund - ECB and Greek public funds. ISK acknowledges the receipt of an OzMalNet fellowship from the EViMalaR network of excellence. GIM is supported by a Discovery Project and Laureate Fellowship from the ARC and a Program Grant from the NHMRC. This work was performed in the framework of the BIOSYS research project, Action KRIPIS, project No MIS-448301 (2013SE01380036) funded by the General Secretariat for Research and Technology, Ministry of Education, Greece and the European Regional Development Fund (Sectoral Operational Programme: Competitiveness and Entrepreneurship, NSRF 2007-2013)/ European Commission.