Journal article
Single cell RNA sequencing of stem cell-derived retinal ganglion cells
M Daniszewski, A Senabouth, QH Nguyen, DE Crombie, SW Lukowski, T Kulkarni, VM Sluch, JS Jabbari, X Chamling, DJ Zack, A Pébay, JE Powell, AW Hewitt
Scientific Data | NATURE PORTFOLIO | Published : 2018
Abstract
We used single cell sequencing technology to characterize the transcriptomes of 1,174 human embryonic stem cell-derived retinal ganglion cells (RGCs) at the single cell level. The human embryonic stem cell line BRN3B-mCherry (A81-H7), was differentiated to RGCs using a guided differentiation approach. Cells were harvested at day 36 and prepared for single cell RNA sequencing. Our data indicates the presence of three distinct subpopulations of cells, with various degrees of maturity. One cluster of 288 cells showed increased expression of genes involved in axon guidance together with semaphorin interactions, cell-extracellular matrix interactions and ECM proteoglycans, suggestive of a more ma..
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Grants
Awarded by Ophthalmic Research Institute of Australia
Funding Acknowledgements
This work was supported by grants from the Sir Edward Dunlop Medical Research Foundation, the Ophthalmic Research Institute of Australia, Retina Australia, the Joan and Peter Clemenger Foundation, and a Philip Neal bequest. AWH & JEP are supported by National Health and Medical Research Council Fellowships, AP by an Australian Research Council Future Fellowship (FT140100047), and MD by an International Postgraduate Award Scholarship from the University of Melbourne. CERA receives Operational Infrastructure Support from the Victorian Government.