Rapid, Loop-Mediated Isothermal Amplification Detection of Celiac Disease Risk Alleles

M Erlichster; JA Tye-Din; MD Varney; E Skafidas; P Kwan

Journal article

Rapid, Loop-Mediated Isothermal Amplification Detection of Celiac Disease Risk Alleles

M Erlichster, JA Tye-Din, MD Varney, E Skafidas, P Kwan

Journal of Molecular Diagnostics | ELSEVIER SCIENCE INC | Published : 2018

DOI: 10.1016/j.jmoldx.2018.01.005

Abstract

Human leukocyte antigen (HLA) genotyping has become a useful investigation in the diagnostic work-up of celiac disease (CD), with utility in risk stratification and screening. However, broad application of this technology has been hindered by the cost and time burden of conventional laboratory-based assays. We have developed and validated CD–loop-mediated isothermal amplification (CD-LAMP), a LAMP assay, which enables rapid identification of the signature CD risk genotypes, HLA-DQ2.5, HLA-DQ8, HLA-DQ2.2, and HLA-DQA1*05. Sample-to-answer is achieved in approximately 65 minutes without DNA purification, thermal cycling, or specialized analytical equipment. CD-LAMP genotyping of samples was 10..

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University of Melbourne Researchers

Jason Tye-Din Author

Stan Skafidas Author

Patrick Kwan Author

Grants

Funding Acknowledgements

Supported by an Australian Postgraduate Award scholarship (M.E.), a Melbourne Neuroscience Institute postgraduate award (M.E.), the Mathison Centenary Fellowship from the University of Melbourne (J.A.T.-D.), and Coeliac Australia (J.A.T.-D.).