Determination of the small RNA GcvB regulon in the Gram-negative bacterial pathogen Pasteurella multocida and identification of the GcvB seed binding region
Emily L Gulliver, Amy Wright, Deanna Deveson Lucas, Marianne Megroz, Oded Kleifeld, Ralf B Schittenhelm, David R Powell, Torsten Seemann, Juergen B Bulitta, Marina Harper, John D Boyce
RNA | COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT | Published : 2018
Pasteurella multocida is a Gram-negative bacterium responsible for many important animal diseases. While a number of P. multocida virulence factors have been identified, very little is known about how gene expression and protein production is regulated in this organism. Small RNA (sRNA) molecules are critical regulators that act by binding to specific mRNA targets, often in association with the RNA chaperone protein Hfq. In this study, transcriptomic analysis of the P. multocida strain VP161 revealed a putative sRNA with high identity to GcvB from Escherichia coli and Salmonella enterica serovar Typhimurium. High-throughput quantitative liquid proteomics was used to compare the proteomes of ..View full abstract
Awarded by Australian Research Council
This work was supported in part by the Australian Research Council discovery project grant number 150103715. E.G. is supported by a Monash University MBio Postgraduate Discovery Scholarship.