DIFFERENTIAL ANTIBODY SCREENING OF CLONED PLASMODIUM-FALCIPARUM SEQUENCES EXPRESSED IN ESCHERICHIA-COLI - PROCEDURE FOR ISOLATION OF DEFINED ANTIGENS AND ANALYSIS OF HUMAN ANTISERA

Abstract

We describe a procedure that uses polyspecific human sera for screening Escherichia coli colonies expressing cloned Plasmodium falciparum cDNA sequences in order to detect colonies that react differentially with different sera. This procedure can be used for two distinct purposes. First, it enables the isolation of clones encoding specified antigenic sequences present in the complex mixture, without purification of either antigens or antibodies by conventional procedures. This requires that the antigen can be expressed in E. coli and that antisera are available that differ substantially in their reactivities to the component of interest. To develop the procedure, we used two polyspecific ser..