Journal article
Supported bilayers formed from different phospholipids on spherical silica substrates
G Gopalakrishnan, I Rouiller, DR Colman, RB Lennox
Langmuir | AMER CHEMICAL SOC | Published : 2009
DOI: 10.1021/la9006982
Abstract
Spherical supported bilayer membranes (SS-BLMs) are very attractive candidates in modern bioanalytics and biorecognition studies. A uniform, facile method of preparing different SS-BLMs on silica beads is reported. Confocal fluorescence microscopy and cryo-TEM imaging have been used to characterize these SS-BLMs. Thermal analysis data and FRAP experiments show that the bilayer properties of the SS-BLM are consistent with those of lipid vesicles from which they are formed. The possibility of modulating the size, lipid type and functionality, and mechanical stability makes these rigid liposomes very attractive candidates in biosensors, drug screening, and gene delivery-related applications. Th..
View full abstractGrants
Awarded by Regenerative Medicine and Nanomedicine Initiative of the Canadian Institutes of Health Research (CIHR)
Funding Acknowledgements
This work is supported by a grant from the Regenerative Medicine and Nanomedicine Initiative of the Canadian Institutes of Health Research (CIHR) to D.R.C. and R.B.L. and a CIHR grant (no. MOP-86693) to I.R. I.R. is recipient of a CIHR New Investigator award. We thank Petr Fiurasek (CSACS) for helping with DSC experiments and Dr. Patricia Yam (MNI&H) for helpful discussions with FRAP measurements.