Journal article
DNA methylation profiling of genomic DNA isolated from urine in diabetic chronic kidney disease: A pilot study
A Lecamwasam, A Sexton-Oates, J Carmody, EI Ekinci, KM Dwyer, R Saffery
Plos One | PUBLIC LIBRARY SCIENCE | Published : 2018
Abstract
Aim To characterise the genomic DNA (gDNA) yield from urine and quality of derived methylation data generated from the widely used Illuminia Infinium MethylationEPIC (HM850K) platform and compare this with buffy coat samples. Background DNA methylation is the most widely studied epigenetic mark and variations in DNA methylation profile have been implicated in diabetes which affects approximately 415 million people worldwide. Methods QIAamp Viral RNA Mini Kit and QIAamp DNA micro kit were used to extract DNA from frozen and fresh urine samples as well as increasing volumes of fresh urine. Matched buffy coats to the frozen urine were also obtained and DNA was extracted from the buffy coats usi..
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Funding Acknowledgements
Dr. Ekinci was supported by a Viertel Clinical Investigatorship, Royal Australasian College of Physicians (RACP) Fellowship, Sir Edward Weary Dunlop Medical Research Foundation and Stroke Foundation research grants. The funders had no role in study design, data collection and analysis, decision to publish or preparation of the manuscript. All the others authors had no financial/funding disclosures.