Time for Multiple Extraction Methods in Proteomics? A Comparison of Three Protein Extraction Methods in the Eustigmatophyte Alga Microchloropsis gaditana CCMP526
Anbarasu Karthikaichamy, Pranali Deore, Vineeta Rai, Dieter Bulach, John Beardall, Santosh Noronha, Sanjeeva Srivastava
OMICS-A JOURNAL OF INTEGRATIVE BIOLOGY | MARY ANN LIEBERT, INC | Published : 2017
Proteomics is a crucial postgenomic biotechnology for functional and systems scale analyses in cell and integrative biology, not to mention clinical and precision medicine research. However, a fundamental requirement for an accurate examination of the protein complement of cells is an efficient method for extracting the proteins. This study reports on the evaluation of three protein extraction methods: trichloroacetic acid (TCA)-acetone, phenol, and TRIzol, in the eustigmatophyte alga Microchloropsis gaditana CCMP526 for proteomic analysis. M. gaditana is a potential candidate for algal-based biofuels. This industrially important strain is also rich in dietary oil and pigments and is used as..View full abstract
Awarded by Reliance Industries Limited, India
The authors acknowledge the technical support from the HR-LC/MS team at SAIF, IIT Bombay. This work was supported by the IITB-Monash Research Academy, IITB, Mumbai, India; Reliance Industries Limited, India (IM-URA0304), and DBT PAN IIT Centre for Bioenergy grant (BT/EB/PANIIT/2012).