Journal article

Koi Herpesvirus Encodes and Expresses a Functional Interleukin-10

Agus Sunarto, Clifford Liongue, Kenneth A McColl, Mathew M Adams, Dieter Bulach, Mark St J Crane, Karel A Schat, Barry Slobedman, Andrew C Barnes, Alister C Ward, Peter J Walker

JOURNAL OF VIROLOGY | AMER SOC MICROBIOLOGY | Published : 2012

Abstract

Koi herpesvirus (KHV) (species Cyprinid herpesvirus 3) ORF134 was shown to transcribe a spliced transcript encoding a 179-amino-acid (aa) interleukin-10 (IL-10) homolog (khvIL-10) in koi fin (KF-1) cells. Pairwise sequence alignment indicated that the expressed product shares 25% identity with carp IL-10, 22 to 24% identity with mammalian (including primate) IL-10s, and 19.1% identity with European eel herpesvirus IL-10 (ahvIL-10). In phylogenetic analyses, khvIL-10 fell in a divergent position from all host IL-10 sequences, indicating extensive structural divergence following capture from the host. In KHV-infected fish, khvIL-10 transcripts were observed to be highly expressed during the ac..

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University of Melbourne Researchers