Cytokine measurement in biological samples after physicochemical treatment for inactivation of biosafety level 4 viral agents.
S Mahanty, R Kalwar, PE Rollin
Journal of Medical Virology | Published : 1999
Physicochemical techniques such as gamma-irradiation, membrane disruption by detergents like sodium dodecyl sulfate (SDS), and fixation with formaldehyde or paraformaldehyde are routinely used to inactivate biological specimens from patients and animals infected with Filoviruses and Arena viruses that must be studied in BSL 4 facilities. The effects of these inactivation techniques on the levels of immunologically active proteins like cytokines and chemokines are not known. Therefore, we investigated the effect of several decontamination techniques on the immunoreactivity and bioactivity of the inflammatory cytokines IL-1beta, IL-6, TNF-alpha, IL-12, and IFN-gamma, the anti-inflammatory cyto..View full abstract