Journal article
Transcriptomic analysis of the activity of a novel polymyxin against Staphylococcus aureus
J Zhao, SE Cheah, KD Roberts, RL Nation, PE Thompson, T Velkov, Z Du, MD Johnson, J Li
Msphere | AMER SOC MICROBIOLOGY | Published : 2016
Abstract
Polymyxin B and colistin are exclusively active against Gram-negative pathogens and have been used in the clinic as a last-line therapy. In this study, we investigated the antimicrobial activity of a novel polymyxin, FADDI-019, against Staphylococcus aureus. MIC and time-kill assays were employed to measure the activity of FADDI-019 against S. aureus ATCC 700699. Cell morphology was examined with scanning electron microscopy (SEM), and cell membrane polarity was measured using flow cytometry. Transcriptome changes caused by FADDI-019 treatment were investigated using transcriptome sequencing (RNA-Seq). Pathway analysis was conducted to examine the mechanism of the antibacterial activity of F..
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Awarded by National Institutes of Health
Funding Acknowledgements
This work, including the efforts of Jian Li and Tony Velkov, was funded by HHS vertical bar NIH vertical bar National Institute of Allergy and Infectious Diseases (NIAID) (R01 AI098771 and R01 AI111965).