Journal article

Transcriptomic Analysis of the Activity of a Novel Polymyxin against Staphylococcus aureus

Jinxin Zhao, Soon-Ee Cheah, Kade D Roberts, Roger L Nation, Philip E Thompson, Tony Velkov, Zongjun Du, Matthew D Johnson, Jian Li

MSPHERE | AMER SOC MICROBIOLOGY | Published : 2016

Abstract

Polymyxin B and colistin are exclusively active against Gram-negative pathogens and have been used in the clinic as a last-line therapy. In this study, we investigated the antimicrobial activity of a novel polymyxin, FADDI-019, against Staphylococcus aureus. MIC and time-kill assays were employed to measure the activity of FADDI-019 against S. aureus ATCC 700699. Cell morphology was examined with scanning electron microscopy (SEM), and cell membrane polarity was measured using flow cytometry. Transcriptome changes caused by FADDI-019 treatment were investigated using transcriptome sequencing (RNA-Seq). Pathway analysis was conducted to examine the mechanism of the antibacterial activity of F..

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Grants

Awarded by HHS \ NIH \ National Institute of Allergy and Infectious Diseases (NIAID)


Awarded by NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES


Funding Acknowledgements

This work, including the efforts of Jian Li and Tony Velkov, was funded by HHS vertical bar NIH vertical bar National Institute of Allergy and Infectious Diseases (NIAID) (R01 AI098771 and R01 AI111965).