Journal article

Near-Atomic Three-Dimensional Mapping for Site-Specific Chemistry of 'Superbugs'

Vahid R Adineh, Ross KW Marceau, Tony Velkov, Jian Li, Jing Fu



Emergence of multidrug resistant Gram-negative bacteria has caused a global health crisis and last-line class of antibiotics such as polymyxins are increasingly used. The chemical composition at the cell surface plays a key role in antibiotic resistance. Unlike imaging the cellular ultrastructure with well-developed electron microscopy, the acquisition of a high-resolution chemical map of the bacterial surface still remains a technological challenge. In this study, we developed an atom probe tomography (APT) analysis approach to acquire mass spectra in the pulsed-voltage mode and reconstructed the 3D chemical distribution of atoms and molecules in the subcellular domain at the near-atomic sc..

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University of Melbourne Researchers


Awarded by Australian National Health & Medical Research Council (NHMRC)

Awarded by Australian Research Council

Awarded by National Institute of Allergy and Infectious Diseases of the National Institutes of Health


Funding Acknowledgements

This study was funded by the Australian National Health & Medical Research Council (NHMRC, APP1046561), Monash University Interdisciplinary Research (IDR) Seed Fund, and the Monash Centre for Atomically Thin Materials (MCATM) for his PhD top-up scholarship. This work was performed in part at the Melbourne Centre for Nanofabrication (MCN), Victorian Node of the Australian National Fabrication Facility (ANFF). The authors acknowledge use of facilities within the Monash Centre for Electron Microscopy (MCEM), and the Advanced Characterisation Facility within the Institute for Frontier Materials (IFM) at Deakin University. This research used equipment funded by the Australian Research Council (LE0882821, LE110100223, and LE120100034). J.L. and T.V. are supported by research grants from the National Institute of Allergy and Infectious Diseases of the National Institutes of Health (R01 AI098771 and AI111965). The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institute of Allergy and Infectious Diseases or the National Institutes of Health. J.L. is an Australian NHMRC Senior Research Fellow. T.V. is an Australian NHMRC Industry Career Development Level 2 Research Fellow. The authors would like to thank Drs. Eric Hanssen and Yu Chen for their assistance in TEM and cryo-TEM imaging.