Journal article
Synergistic killing of NDM-producing MDR Klebsiella pneumoniae by two 'old' antibiotics-polymyxin B and chloramphenicol
NA Rahim, SE Cheah, MD Johnson, H Yu, HE Sidjabat, J Boyce, MS Butler, MA Cooper, J Fu, DL Paterson, RL Nation, PJ Bergen, T Velkov, J Li
Journal of Antimicrobial Chemotherapy | OXFORD UNIV PRESS | Published : 2015
DOI: 10.1093/jac/dkv135
Abstract
Objectives: Combination therapy is an important option in the fight against Gram-negative 'superbugs'. This study systematically investigated bacterial killing and the emergence of polymyxin resistance with polymyxin B and chloramphenicol combinations used against New Delhi metallo-β-lactamase (NDM)-producing MDR Klebsiella pneumoniae. Methods: Four NDM-producing K. pneumoniae strains were employed. The presence of genes conferring resistance to chloramphenicol was examined by PCR. Time-kill studies (inocula ~106 cfu/mL) were conducted using various clinically achievable concentrations of each antibiotic (range: Polymyxin B, 0.5-2 mg/L; chloramphenicol, 4-32 mg/L), with real-time population ..
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Awarded by Foundation for the National Institutes of Health
Funding Acknowledgements
This study was partially funded by the Australian National Health and Medical Research Council (NHMRC APP1046561). J. L., R. L. N. and T. V. are supported by research grants from the National Institute of Allergy and Infectious Diseases of the National Institutes of Health [R01 A1098771 (J. L., R. L. N. and T. V.) and R01 AI111965 (J. L. and T. V.)]. M. A. C. is an Australian NHMRC Principal Research Fellow. T. V. is an Australian NHMRC Industry Career Development Research Fellow. J. L. is an Australian NHMRC Senior Research Fellow.