Journal article

Analysis of Phosphopeptide Changes as Spermatozoa Acquire Functional Competence in the Epididymis Demonstrates Changes in the Post-translational Modification of Izumo1

Mark A Baker, Louise Hetherington, Anita Weinberg, Nenad Naumovski, Tony Velkov, Matthias Pelzing, Sebastiaan Dolman, Mark R Condina, R John Aitken

JOURNAL OF PROTEOME RESEARCH | AMER CHEMICAL SOC | Published : 2012

Abstract

Spermatozoa are functionally inert when they emerge from the testes. Functional competence is conferred upon these cells during a post-testicular phase of sperm maturation in the epididymis. Remarkably, this functional transformation of epididymal spermatozoa occurs in the absence of nuclear gene transcription or protein translation. To understand the cellular mechanisms underpinning epididymal maturation, we have performed a label-free, MS-based, comparative quantification of peptides from caput, corpus and caudal epididymal spermatozoa. In total, 68 phosphopeptide changes could be detected during epididymal maturation corresponding to the identification of 22 modified proteins. Included in..

View full abstract

University of Melbourne Researchers