Non-random Lymphocyte Distribution Among Virus-Infected Cells of the Respiratory Tract
Rajeev Rudraraju, Robert E Sealy, Sherri L Surman, Paul G Thomas, Barry H Dayton, Julia L Hurwitz
VIRAL IMMUNOLOGY | MARY ANN LIEBERT, INC | Published : 2013
The rules of T cell positioning within virus-infected respiratory tract tissues are poorly understood. We therefore marked cervical lymph node or spleen cells from Sendai virus (SeV) primed mice and transferred lymphocytes to animals infected with SeV expressing an enhanced green fluorescent protein (SeV-eGFP). Confocal imaging showed that when T cells entered a field of infected respiratory tract epithelium, they assumed a spatial distribution that maximized distances between each donor cell and its nearest neighbor. We therefore hypothesized that lymphocytes repelled one another by altering their chemokine/cytokine microenvironment. Subsequent in vitro tests confirmed that when SeV-primed ..View full abstract
Awarded by NIH
Awarded by NATIONAL CANCER INSTITUTE
Awarded by NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
We thank the Animal Resources Center at St. Jude for animal care. Research was funded in part by NIH P30 CA21765, We thank Victoria Frohlich and Jamshid Temirov of the St. Jude imaging core for assistance with confocal microscopy. P01 AI054955, R01 AI088729 and the American Lebanese Syrian Associated Charities (ALSAC).