Journal article

ABC-F Proteins Mediate Antibiotic Resistance through Ribosomal Protection

Liam KR Sharkey, Thomas A Edwards, Alex J O'Neill



Members of the ABC-F subfamily of ATP-binding cassette proteins mediate resistance to a broad array of clinically important antibiotic classes that target the ribosome of Gram-positive pathogens. The mechanism by which these proteins act has been a subject of long-standing controversy, with two competing hypotheses each having gained considerable support: antibiotic efflux versus ribosomal protection. Here, we report on studies employing a combination of bacteriological and biochemical techniques to unravel the mechanism of resistance of these proteins, and provide several lines of evidence that together offer clear support to the ribosomal protection hypothesis. Of particular note, we show ..

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University of Melbourne Researchers


Awarded by BBSRC-DTG studentship

Awarded by Biotechnology and Biological Sciences Research Council

Funding Acknowledgements

This work was funded by BBSRC-DTG studentship BB/F016603/1 awarded to the University of Leeds. The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.