Journal article
Allergen-encoding bone marrow transfer inactivates allergic T cell responses, alleviating airway inflammation
J AL-Kouba, AN Wilkinson, MR Starkey, R Rudraraju, RB Werder, X Liu, SC Law, JC Horvat, JF Brooks, GR Hill, JM Davies, S Phipps, PM Hansbro, RJ Steptoe
Jci Insight | AMER SOC CLINICAL INVESTIGATION INC | Published : 2017
Open access
Abstract
Memory Th2 cell responses underlie the development and perpetuation of allergic diseases. Because these states result from immune dysregulation, established Th2 cell responses represent a significant challenge for conventional immunotherapies. New approaches that overcome the detrimental effects of immune dysregulation are required. We tested whether memory Th2 cell responses were silenced using a therapeutic approach where allergen expression in DCs is transferred to sensitized recipients using BM cells as a vector for therapeutic gene transfer. Development of allergen-specific Th2 responses and allergen-induced airway inflammation was blocked by expression of allergen in DCs. Adoptive tran..
View full abstractGrants
Awarded by Australian Research Council
Funding Acknowledgements
This work was supported by NHMRC Project grant GNT1013066 (RJS, JMD) and Asthma Foundation of Queensland (RJS). RJS was a recipient of Australian Research Council Future Fellowship (FT110100372). MRS was a recipient of an NHMRC Early Career Fellowship (GNT1073317). PMH was a recipient of an NHMRC Principal Research Fellowship (GNT1079187) and a Brawn Research Fellowship. SP was a recipient of Australian Research Council Future Fellowship (FT130100518). GRH is an Australian National Health and Medical Research Council senior principal research fellow. The authors acknowledge the technical contribution of Tracy Doan, Chantelle Reid, and Ryan Galea and members of TRI Flow Cytometry, Microscopy and Biological Resource Facilities to experimental studies.