Journal article

Bile canalicular dynamics in hepatocyte sandwich cultures

Raymond Reif, Johan Karlsson, Georgia Guenther, Lynette Beattie, David Wrangborg, Seddik Hammad, Brigitte Begher-Tibbe, Amruta Vartak, Simone Melega, Paul M Kaye, Jan G Hengstler, Mats Jirstrand

Archives of Toxicology | SPRINGER HEIDELBERG | Published : 2015

Abstract

Many substances are hepatotoxic due to their ability to cause intrahepatic cholestasis. Therefore, there is a high demand for in vitro systems for the identification of cholestatic properties of new compounds. Primary hepatocytes cultivated in collagen sandwich cultures are known to establish bile canaliculi which enclose secreted biliary components. Cholestatic compounds are mainly known to inhibit bile excretion dynamics, but may also alter canalicular volume, or hepatocellular morphology. So far, techniques to assess time-resolved morphological changes of bile canaliculi in sandwich cultures are not available. In this study, we developed an automated system that quantifies dynamics of bil..

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University of Melbourne Researchers

Grants

Awarded by European Commission


Awarded by Virtual Liver Network initiative of the German Federal Ministry of Education and Research


Awarded by UK Medical Research Council


Awarded by Medical Research Council


Funding Acknowledgements

This work was partially funded by the European Commission Seventh Framework Programme CANCERSYS (FP7-2008-2011; Grant #223188), NOTOX (FP7-2007-2013; Grant #267038), DETECTIVE (FP7-2007-2013; Grant #266838), the Virtual Liver Network initiative of the German Federal Ministry of Education and Research (Grant #03157399) and by the UK Medical Research Council (Grant #G0802620).