IL-10-Producing Th1 Cells and Disease Progression Are Regulated by Distinct CD11c( ) Cell Populations during Visceral Leishmaniasis
Benjamin MJ Owens, Lynette Beattie, John WJ Moore, Najmeeyah Brown, Jason L Mann, Jane E Dalton, Asher Maroof, Paul M Kaye
PLoS Pathogens | PUBLIC LIBRARY SCIENCE | Published : 2012
IL-10 is a critical regulatory cytokine involved in the pathogenesis of visceral leishmaniasis caused by Leishmania donovani and clinical and experimental data indicate that disease progression is associated with expanded numbers of CD4⁺ IFNγ⁺ T cells committed to IL-10 production. Here, combining conditional cell-specific depletion with adoptive transfer, we demonstrate that only conventional CD11c(hi) DCs that produce both IL-10 and IL-27 are capable of inducing IL-10-producing Th1 cells in vivo. In contrast, CD11c(hi) as well as CD11c(int/lo) cells isolated from infected mice were capable of reversing the host protective effect of diphtheria toxin-mediated CD11c⁺ cell depletion. This was ..View full abstract
Awarded by Medical Research Council
This work was supported by grants (to P. M. K.) from the UK Medical Research Council (http://www.mrc.ac.uk/) and the Wellcome Trust (http://www.wellcome.ac.uk/) and by a MRC Doctoral Training Award (to B.M.J.O.). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.