Hormone-sensing cells require Wip1 for paracrine stimulation in normal and premalignant mammary epithelium
Gerard A Tarulli, Duvini De Silva, Victor Ho, Kamini Kunasegaran, Kakaly Ghosh, Bryan C Tan, Dmitry V Bulavin, Alexandra M Pietersen
Breast Cancer Research | BMC | Published : 2013
INTRODUCTION: The molecular circuitry of different cell types dictates their normal function as well as their response to oncogene activation. For instance, mice lacking the Wip1 phosphatase (also known as PPM1D; protein phosphatase magnesium-dependent 1D) have a delay in HER2/neu (human epidermal growth factor 2), but not Wnt1-induced mammary tumor formation. This suggests a cell type-specific reliance on Wip1 for tumorigenesis, because alveolar progenitor cells are the likely target for transformation in the MMTV(mouse mammary tumor virus)-neu but not MMTV-wnt1 breast cancer model. METHODS: In this study, we used the Wip1-knockout mouse to identify the cell types that are dependent on Wip1..View full abstract
Awarded by Agency for Science, Technology and Research (A*STAR Singapore)
We thank Jen Nee Goh for technical assistance with optimizing the methods. We are grateful for expert advice on MEC isolation, FACS, and comments on the manuscript from Matthew Smalley. This work was supported by funding from the National Cancer Centre Singapore, Duke-NUS Graduate Medical School, and a grant from the Agency for Science, Technology and Research (A*STAR Singapore, SSCC09/014) to AP and DB.