Journal article

The &ITPlasmodium falciparum &ITtranscriptome in severe malaria reveals altered expression of genes involved in important processes including surface antigen-encoding &ITvar &ITgenes

Gerry Q Tonkin-Hill, Leily Trianty, Rintis Noviyanti, Hanh HT Nguyen, Boni F Sebayang, Daniel A Lampah, Jutta Marfurt, Simon A Cobbold, Janavi S Rambhatla, Malcolm J McConville, Stephen J Rogerson, Graham Brown, Karen P Day, Ric N Price, Nicholas M Anstey, Anthony T Papenfuss, Michael F Duffy

PLOS BIOLOGY | PUBLIC LIBRARY SCIENCE | Published : 2018

Abstract

Within the human host, the malaria parasite Plasmodium falciparum is exposed to multiple selection pressures. The host environment changes dramatically in severe malaria, but the extent to which the parasite responds to-or is selected by-this environment remains unclear. From previous studies, the parasites that cause severe malaria appear to increase expression of a restricted but poorly defined subset of the PfEMP1 variant, surface antigens. PfEMP1s are major targets of protective immunity. Here, we used RNA sequencing (RNAseq) to analyse gene expression in 44 parasite isolates that caused severe and uncomplicated malaria in Papuan patients. The transcriptomes of 19 parasite isolates assoc..

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Grants

Awarded by National Health and Medical Research Council


Awarded by Wellcome Trust


Funding Acknowledgements

National Health and Medical Research Council https://www.nhmrc.gov.au/ (grant number 1007954) project grant received by MFD and ATP. The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. National Health and Medical Research Council https://www.nhmrc.gov.au/ (grant number 1054618) program grant received by ATP. The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. National Health and Medical Research Council https://www.nhmrc.gov.au/ (grant number 1042072). Practitioner Fellowship received by NMA. The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Wellcome Trust https://wellcome.ac.uk (grant number 200909). Senior Fellow in Clinical Science received by RNP. The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Australian Department of Foreign Affairs and Trade http://dfat.gov.au. The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Victorian State Government Operational Infrastructure Support and Australian Government NHMRC IRIISS received by ATP. The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.