The performance of three immune assays to assess the serological status of cattle experimentally exposed to Mycoplasma bovis
ML Schibrowski, TS Barnes, NK Wawegama, ME Vance, PF Markham, PD Mansell, MS Marenda, A Kanci, J Perez-Casal, GF Browning, JS Gibson, TJ Mahony
Veterinary Sciences | MDPI | Published : 2018
© 2018 by the authors. Mycoplasma bovis is associated with several clinical syndromes of cattle. Currently, limited information is available on the sensitivity (Se) and specificity (Sp) of serological assays used for the detection of M. bovis-specific antibodies. Consequently, it is difficult to critically evaluate the outcomes of studies that use these assays. Therefore, the current study used bovine sera sourced from M. bovis exposure studies from three countries to estimate the Se and Sp of two commercial M. bovis enzyme-linked immunosorbent assays (ELISA), BIO K302 and BIO K260, and Western blotting. Western blotting had the highest Se estimate of 74% (95% confidence interval (CI): 16-98..View full abstract
Awarded by Meat and Livestock Australia
This project was jointly supported by the Department of Agriculture and Fisheries and The University of Queensland and by grant B.FLT.0225 from Meat and Livestock Australia with matching funds provided by the Australian Government. MLS was a recipient of an Australian Postgraduate Award from the Australian Government. The authors are indebted to Robin A.J. Nicholas and Roger D. Ayling (Animal and Plant Health Agency (Weybridge), Addlestone, Surrey, UK) for provision of the serum samples from English cattle used in this study. The authors also gratefully acknowledge Konrad Sachse (Federal Research Institute for Animal Health, Institute of Molecular Pathogenesis, Jena, Germany) for providing the monoclonal antibodies used in this study.