Contribution of NK cell education to both direct and anti-HIV-1 antibodydependent NK cell functions

Abstract

Antibody Fc-dependent functions are linked to prevention and control of HIV-1 infection. Basic NK cell biology is likely key to understanding the contributions that anti-HIV-1 antibody-dependent NK cell activation and cytolysis make to HIV-1 susceptibility and disease progression. The importance of NK cell education through inhibitory receptors specific for self-HLA-I in determining the potency of anti-HIV-1 antibody-mediated NK cell activation and cytolysis is controversial. To address this issue more definitively, we utilized HLA-I genotyping, flow cytometry staining panels, and cytolysis assays to assess the functionality of educated and noneducated peripheral blood NK cells. We now demon..