Design-based stereological methods for estimating numbers of glomerular podocytes
Victor G Puelles, Rebecca N Douglas-Denton, Luise Cullen-McEwen, Bridgette J McNamara, Firuz Saliha, Jinhua Li, Michael D Hughson, Wendy E Hoy, Jens R Nyengaard, John F Bertram
ANNALS OF ANATOMY-ANATOMISCHER ANZEIGER | ELSEVIER GMBH, URBAN & FISCHER VERLAG | Published : 2014
The podocyte depletion hypothesis has emerged as a unifying concept in glomerular pathology. According to this hypothesis podocyte depletion may be absolute (decrease in number of healthy mature podocytes), relative (fewer podocytes per unit of glomerular volume) or involve alterations to the specialized podocyte architecture (such as foot process effacement). To study and understand podocyte depletion it is important to be able to accurately and precisely count these cells. Here we present new design-based stereological methods for estimating podocyte number in individual glomeruli of known volume, and in average glomeruli. Both methods involve serial histological sectioning, triple label i..View full abstract
Awarded by NHMRC of Australia
Awarded by Villum Fonden
The authors acknowledge the facilities, scientific and technical assistance of Monash Micro Imaging and the Histology Platform at Monash University, Victoria, Australia. VGP received a Monash Research Graduate School Scholarship and a Faculty of Medicine International Postgraduate Scholarship to support his PhD candidature. This work is funded by the NHMRC of Australia (grant number: 606619) and the Center for Stochastic Geometry and Advanced Bioimaging (CSGB) is supported by the Villum Foundation.