Journal article

Fitness cost of mcr-1-mediated polymyxin resistance in Klebsiella pneumoniae

Sue C Nang, Faye C Morris, Michael J McDonald, Mei-Ling Han, Jiping Wang, Richard A Strugnell, Tony Velkov, Jian Li

JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY | OXFORD UNIV PRESS | Published : 2018

Abstract

Objectives: The discovery of mobile colistin resistance mcr-1, a plasmid-borne polymyxin resistance gene, highlights the potential for widespread resistance to the last-line polymyxins. In the present study, we investigated the impact of mcr-1 acquisition on polymyxin resistance and biological fitness in Klebsiella pneumoniae. Methods: K. pneumoniae B5055 was used as the parental strain for the construction of strains carrying vector only (pBBR1MCS-5) and mcr-1 recombinant plasmids (pmcr-1). Plasmid stability was determined by serial passaging for 10 consecutive days in antibiotic-free LB broth, followed by patching on gentamicin-containing and antibiotic-free LB agar plates. Lipid A was ana..

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Grants

Awarded by National Institute of Allergy and Infectious Diseases of the National Institutes of Health


Awarded by Australian National Health and Medical Research Council (NHMRC)


Awarded by NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES


Funding Acknowledgements

This study was supported by a grant from the National Institute of Allergy and Infectious Diseases of the National Institutes of Health (R01 AI111965) and an Australian National Health and Medical Research Council (NHMRC) project grant (1064896). J. L. is an NHMRC Senior Research Fellow. T. V. is an Australian NHMRC Career Development Research Fellow.