Transcriptome analysis of mammary epithelial cell gene expression reveals novel roles of the extracellular matrix.
Stephen S Wanyonyi, Amit Kumar, Ryan Du Preez, Christophe Lefevre, Kevin R Nicholas
Biochem Biophys Rep | Published : 2017
BACKGROUND: The unique lactation strategy of the tammar wallaby (Macropus eugeni) has been invaluable in evaluating the role of lactogenic hormones and the extracellular matrix (ECM) in the local control of mammary gland function. However molecular pathways through which hormones and ECM exert their effect on wallaby mammary gland function remain unclear. This study undertakes transcriptome analysis of wallaby mammary epithelial cells (WallMEC) following treatment with mammary ECM from two distinct stages of lactation. METHODS: WallMEC from MID lactation mammary glands were cultured on ECM from MID or LATE lactation and treated for 5 days with 1 μg/ml cortisol, 1 μg/ml insulin, 0.2 µg/ml pro..View full abstract