Hormonal regulation of platypus Beta-lactoglobulin and monotreme lactation protein genes
Ashwantha Kumar Enjapoori, Christophe M Lefevre, Kevin R Nicholas, Julie A Sharp
GENERAL AND COMPARATIVE ENDOCRINOLOGY | ACADEMIC PRESS INC ELSEVIER SCIENCE | Published : 2017
Endocrine regulation of milk protein gene expression in marsupials and eutherians is well studied. However, the evolution of this complex regulation that began with monotremes is unknown. Monotremes represent the oldest lineage of extant mammals and the endocrine regulation of lactation in these mammals has not been investigated. Here we characterised the proximal promoter and hormonal regulation of two platypus milk protein genes, Beta-lactoglobulin (BLG), a whey protein and monotreme lactation protein (MLP), a monotreme specific milk protein, using in vitro reporter assays and a bovine mammary epithelial cell line (BME-UV1). Insulin and dexamethasone alone provided partial induction of MLP..View full abstract
This research was supported by in-house funds from the Deakin University, Australia.