A guide to integrating immunohistochemistry and chemical imaging

DP Bishop; N Cole; T Zhang; PA Doble; DJ Hare

Journal article

A guide to integrating immunohistochemistry and chemical imaging

DP Bishop, N Cole, T Zhang, PA Doble, DJ Hare

Chemical Society Reviews | ROYAL SOC CHEMISTRY | Published : 2018

DOI: 10.1039/c7cs00610a

Abstract

Chemical imaging provides new insight into the fundamental atomic, molecular, and biochemical composition of tissue and how they are interrelated in normal physiology. Visualising and quantifying products of pathogenic reactions long before structural changes become apparent also adds a new dimension to understanding disease pathogenesis. While chemical imaging in isolation is somewhat limited by the nature of information it can provide (e.g. peptides, metals, lipids, or functional groups), integrating immunohistochemistry allows simultaneous, targeted imaging of biomolecules while also mapping tissue composition. Together, this approach can provide invaluable information on the inner workin..

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University of Melbourne Researchers

Dominic Hare Author

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Grants

Awarded by Agilent Technologies

Funding Acknowledgements

DPB is supported by an Australian Research Council Discovery Early Career Researcher Award (DE180100194). PAD is the recipient of an Australian Research Council Discovery Project (DP170100036). TZ is supported by a Parkinson's Victoria PhD Scholarship. DJH is supported by an Australian National Health and Medical Research Council Industry Career Development Fellowship (1122981) in partnership with Agilent Technologies. Due to space limitations, this Tutorial Review could not give appropriate credit to all research in this field, and we wish to acknowledge to the authors of the many great works that were not cited directly.