Recovery of the sub-basal nerve plexus and superficial nerve terminals after corneal epithelial injury in mice

LE Downie; C Naranjo Golborne; M Chen; N Ho; C Hoac; D Liyanapathirana; C Luo; RB Wu; HR Chinnery

Journal article

Recovery of the sub-basal nerve plexus and superficial nerve terminals after corneal epithelial injury in mice

LE Downie, C Naranjo Golborne, M Chen, N Ho, C Hoac, D Liyanapathirana, C Luo, RB Wu, HR Chinnery

Experimental Eye Research | ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD | Published : 2018

DOI: 10.1016/j.exer.2018.03.012

Abstract

Our aim was to compare regeneration of the sub-basal nerve plexus (SBNP) and superficial nerve terminals (SNT) following corneal epithelial injury. We also sought to compare agreement when quantifying nerve parameters using different image analysis techniques. Anesthetized, female C57BL/6 mice received central 1-mm corneal epithelial abrasions. Four-weeks post-injury, eyes were enucleated and processed for PGP9.5 to visualize the corneal nerves using wholemount immunofluorescence staining and confocal microscopy. The percentage area of the SBNP and SNT were quantified using: ImageJ automated thresholds, ImageJ manual thresholds and manual tracings in NeuronJ. Nerve sum length was quantified ..

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University of Melbourne Researchers

Laura Downie Author

Holly Chinnery Author

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Grants

Awarded by National Health and Medical Research Council

Funding Acknowledgements

This work was supported by National Health and Medical Research Council (NHMRC) Project Grants [APP1042612 and APP1126450]. The sponsor had no role in the design or conduct of this research. No conflicting relationship exists for any author in relation to this work.