Journal article
Ionophore and Biometal Modulation of P-glycoprotein Expression and Function in Human Brain Microvascular Endothelial Cells
MP McInerney, I Volitakis, AI Bush, WA Banks, JL Short, JA Nicolazzo
Pharmaceutical Research | SPRINGER/PLENUM PUBLISHERS | Published : 2018
Abstract
Purpose: Biometals such as zinc and copper have been shown to affect tight junction expression and subsequently blood-brain barrier (BBB) integrity. Whether these biometals also influence the expression and function of BBB transporters such as P-glycoprotein (P-gp) however is currently unknown. Methods: Using the immortalised human cerebral microvascular endothelial (hCMEC/D3) cell line, an in-cell western assay (alongside western blotting) assessed relative P-gp expression after treatment with the metal ionophore clioquinol and biometals zinc and copper. The fluorescent P-gp substrate rhodamine-123 was employed to observe functional modulation, and inductively coupled plasma mass spectromet..
View full abstractGrants
Awarded by Judith Jane Mason and Harold Stannett Williams Memorial Foundation
Funding Acknowledgements
The studies completed within this publication were funded by the following sources; NHMRC Project APP1048855; the Mason Foundation and the Bethlehem Griffiths Research Foundation. Mitchell P. McInerney is supported by an Australian Government Research Training Program Scholarship.