Journal article
Cell Type of Origin Dictates the Route to Pluripotency
CM Nefzger, FJ Rossello, J Chen, X Liu, AS Knaupp, J Firas, JM Paynter, J Pflueger, S Buckberry, SM Lim, B Williams, S Alaei, K Faye-Chauhan, E Petretto, SK Nilsson, R Lister, M Ramialison, DR Powell, OJL Rackham, JM Polo
Cell Reports | CELL PRESS | Published : 2017
Abstract
Our current understanding of induced pluripotent stem cell (iPSC) generation has almost entirely been shaped by studies performed on reprogramming fibroblasts. However, whether the resulting model universally applies to the reprogramming process of other cell types is still largely unknown. By characterizing and profiling the reprogramming pathways of fibroblasts, neutrophils, and keratinocytes, we unveil that key events of the process, including loss of original cell identity, mesenchymal to epithelial transition, the extent of developmental reversion, and reactivation of the pluripotency network, are to a large degree cell-type specific. Thus, we reveal limitations for the use of fibroblas..
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Awarded by Medical Research Council
Funding Acknowledgements
We thank the high-quality cell sorting service and technical input provided by the Monash University FlowCore facility. Furthermore, the authors thank the ACRF Centre for Cancer Genomic Medicine at the MHTP Medical Genomics Facility for assistance with next generation library preparation and Illumina sequencing. This work was supported by National Health and Medical Research Council project grant APP1051309 to J.M. Polo. A.S.K. was supported by National Health and Medical Research Council Early Career Fellowship APP1092280. X.L. and S.A. were supported by a Monash International Postgraduate Research Scholarship and a Monash Graduate Scholarship. J.M. Polo and R.L. were supported by Silvia and Charles Viertel Senior Medical Research Fellowships. O.R. was supported by National Medical Research Council (Singapore) grant OFYIRG16may050. Owen Rackham and Jose Polo are founders and directors of Cell Mogrify. The other authors do not declare any conflict of interest.