A comparison of cochlear distribution and glucocorticoid receptor activation in local and systemic dexamethasone drug delivery regimes
Nathan J Creber, Hayden T Eastwood, Amy J Hampson, Justin Tan, Stephen J O'Leary
HEARING RESEARCH | ELSEVIER SCIENCE BV | Published : 2018
Local and systemically delivered glucocorticoids are commonly administered to protect the cochlea against damage associated with a variety of insults. There is reason to believe that dexamethasone administered by these routes may arrive at cochlear target sites via different pathways. Clinically, there is a lack of clarity as to which route is more effective in any specific circumstance. This study explores dexamethasone distribution within the guinea pig cochlea following local and systemic delivery methods. A combination of mass spectroscopy and immunohistochemistry were employed to compare both perilymph distribution, tissue uptake and receptor activation. Local administration of dexameth..View full abstract
The authors would like to thank Samantha Moreland for assistance with animal husbandry, Sam lssah for assistance with mass spectroscopy, and Elisha Thomas for advice on perilymph sampling. This work was supported by a scholarship from the Garnett Passe and Rodney Williams Memorial Foundation, Melbourne, Australia.