Journal article
Gene expression and pathways underlying form deprivation myopia in the Guinea pig sclera
N Srinivasalu, SA McFadden, C Medcalf, L Fuchs, J Chung, G Philip, A Richardson, M Riaz, PN Baird
Investigative Ophthalmology and Visual Science | ASSOC RESEARCH VISION OPHTHALMOLOGY INC | Published : 2018
Abstract
PURPOSE. Posterior scleral remodeling accompanies myopia. In guinea pigs developing myopia, the region around the optic nerve (peripapillary zone, PPZ) rapidly expands followed by inhibition in eye size in the periphery. We studied the differential gene expression in the sclera that accompanies these changes. METHODS. Guinea pigs were form-deprived (FD) for 2 weeks to induce myopia, while the fellow eye served as a control. After 2 weeks, the PPZ and the peripheral temporal sclera were isolated in representative animals to extract the RNA. RNA sequencing was undertaken using an Illumina HiSeq 2000, with differential expression analyzed using Voom and pathways analyzed using the Ingenuity Pat..
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Grants
Awarded by Sam and Rie Bloomfield Foundation
Funding Acknowledgements
Supported by National Health and Medical Research Council of Australia Senior Research Fellowship 1028444 and 1138585 (PNB) and grants from the Coal Port Authority and Hunter Medical Research Institute (SAM), also a Research Higher Degree Studentship and Melbourne International fee remission scholarship from the University of Melbourne Australia (NS). The Centre for Eye Research Australia (CERA) receives Operational Infrastructure Support from the Victorian Government.