Journal article

Drosophila melanogaster Guk-holder interacts with the Scribbled PDZ1 domain and regulates epithelial development with Scribbled and Discs Large

Sofia Caria, Charlene M Magtoto, Tinaz Samiei, Marta Portela, Krystle YB Lim, Jing Yuan How, Bryce Z Stewart, Patrick O Humbert, Helena E Richardson, Marc Kvansakul



Epithelial cell polarity is controlled by components of the Scribble polarity module, and its regulation is critical for tissue architecture and cell proliferation and migration. In Drosophila melanogaster, the adaptor protein Guk-holder (Gukh) binds to the Scribbled (Scrib) and Discs Large (Dlg) components of the Scribble polarity module and plays an important role in the formation of neuromuscular junctions. However, Gukh's role in epithelial tissue formation and the molecular basis for the Scrib-Gukh interaction remain to be defined. We now show using isothermal titration calorimetry that the Scrib PDZ1 domain is the major site for an interaction with Gukh. Furthermore, we defined the str..

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Awarded by National Health and Medical Research Council Australia Project

Awarded by Australian Research Council Fellowship

Awarded by Cancer Council Victoria

Funding Acknowledgements

This work was supported in whole or part by National Health and Medical Research Council Australia Project Grant APP1103871 (to M. K., P. O. H., and H. E. R.), Fellowship APP1079133 (to P. O. H.), and APP1020056 (to H. E. R.); Australian Research Council Fellowship FT130101349 (to M. K.); Cancer Council Victoria grant funding (to M. P.) and Project Grant APP1041817 (to H. E. R.); La Trobe University Scholarship (to C. M. M. and K. Y. B. L.), and La Trobe University and La Trobe School of Molecular Science funding (to H. E. R.). The authors declare that they have no conflicts of interest with the contents of this article.