Journal article

Characterization of isolated liver sinusoidal endothelial cells for liver bioengineering

AM Dingle, KK Yap, Y-W Gerrand, CJ Taylor, E Keramidaris, Z Lokmic, AM Kong, HL Peters, WA Morrison, GM Mitchell

ANGIOGENESIS | SPRINGER | Published : 2018

Abstract

BACKGROUND: The liver sinusoidal capillaries play a pivotal role in liver regeneration, suggesting they may be beneficial in liver bioengineering. This study isolated mouse liver sinusoidal endothelial cells (LSECs) and determined their ability to form capillary networks in vitro and in vivo for liver tissue engineering purposes. METHODS AND RESULTS: In vitro LSECs were isolated from adult C57BL/6 mouse livers. Immunofluorescence labelling indicated they were LYVE-1+/CD32b+/FactorVIII+/CD31-. Scanning electron microscopy of LSECs revealed the presence of characteristic sieve plates at 2 days. LSECs formed tubes and sprouts in the tubulogenesis assay, similar to human microvascular endothelia..

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Grants

Awarded by National Health and Medical Research Council of Australia


Funding Acknowledgements

This research was funded by National Health and Medical Research Council of Australia Project Grants (1023187 and 1125233); St Vincent's Hospital Melbourne, Research Endowment Fund; the Australian Catholic University; the Stafford Fox Foundation, Australia; and the Victorian State Government's Department of Innovation, Industry and Regional Development's Operational Infrastructure Support Program. AMD was supported by an Australian Post Graduate Award. KKY is supported by scholarships from the National Health and Medical Research Council of Australia; Australia and New Zealand Hepatic, Pancreatic, and Biliary Association; and St Vincent's Institute Foundation.