Journal article
Proteomic identification of galectin- 11 and 14 ligands from Haemonchus contortus
D Sakthivel, J Swan, S Preston, MD Shakif-Azam, P Faou, Y Jiao, R Downs, H Rajapaksha, R Gasser, D Piedrafita, T Beddoe
Peerj | PEERJ INC | Published : 2018
DOI: 10.7717/peerj.4510
Abstract
Haemonchus contortus is the most pathogenic nematode of small ruminants. Infection in sheep and goats results in anaemia that decreases animal productivity and can ultimately cause death. The involvement of ruminant-specific galectin-11 (LGALS-11) and galectin-14 (LGALS-14) has been postulated to play important roles in protective immune responses against parasitic infection; however, their ligands are unknown. In the current study, LGALS-11 and LGALS-14 ligands in H. contortus were identified from larval (L4) and adult parasitic stages extracts using immobilised LGALS-11 and LGALS-14 affinity column chromatography and mass spectrometry. Both LGALS-11 and LGALS-14 bound more putative protein..
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Funding Acknowledgements
Dhanasekaran Sakthivel was supported by Victoria India Doctoral Fellowship from the Department of State Development, Business and Innovation (DSDBI), Victoria, Australia and Australia India Institute (AII). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.