Journal article
INSL5 activates multiple signalling pathways and regulates GLP-1 secretion in NCI-H716 cells
SY Ang, BA Evans, DP Poole, R Bron, JJ DiCello, RAD Bathgate, M Kocan, DS Hutchinson, RJ Summers
Journal of Molecular Endocrinology | BIOSCIENTIFICA LTD | Published : 2018
DOI: 10.1530/JME-17-0152
Abstract
Insulin-like peptide 5 (INSL5) is a newly discovered gut hormone expressed in colonic enteroendocrine L-cells but little is known about its biological function. Here, we show using RT-qPCR and in situ hybridisation that Insl5 mRNA is highly expressed in the mouse colonic mucosa, colocalised with proglucagon immunoreactivity. In comparison, mRNA for RXFP4 (the cognate receptor for INSL5) is expressed in various mouse tissues, including the intestinal tract. We show that the human enteroendocrine L-cell model NCI-H716 cell line, and goblet-like colorectal cell lines SW1463 and LS513 endogenously express RXFP4. Stimulation of NCI-H716 cells with INSL5 produced phosphorylation of ERK1/2 (Thr202/..
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Awarded by Australian Research Council
Funding Acknowledgements
This work is supported by an Australian National Health and Medical Research Council (NHMRC) program grant (1055134; R J S). S Y A is a recipient of the Monash Graduate Scholarship (MGE) and Monash International Postgraduate Research Scholarship (MIPRS). D S H is supported by a NHMRC Career Development Fellowship (545952). Research at the Florey was supported by an ARC Linkage grant to RADB (LP120100654) and by the Victorian Government Operational Infrastructure Support Program. R A D B is supported by an NHMRC Research Fellowship.