Cholinergic Submucosal Neurons Display Increased Excitability Following in Vivo Cholera Toxin Exposure in Mouse Ileum
Candice Fung, Katerina Koussoulas, Petra Unterweger, Andrew M Allen, Joel C Bornstein, Jaime PP Foong
FRONTIERS IN PHYSIOLOGY | FRONTIERS MEDIA SA | Published : 2018
Cholera-induced hypersecretion causes dehydration and death if untreated. Cholera toxin (CT) partly acts via the enteric nervous system (ENS) and induces long-lasting changes to enteric neuronal excitability following initial exposure, but the specific circuitry involved remains unclear. We examined this by first incubating CT or saline (control) in mouse ileal loops in vivo for 3.5 h and then assessed neuronal excitability in vitro using Ca2+ imaging and immunolabeling for the activity-dependent markers cFos and pCREB. Mice from a C57BL6 background, including Wnt1-Cre;R26R-GCaMP3 mice which express the fluorescent Ca2+ indicator GCaMP3 in its ENS, were used. Ca2+-imaging using this mouse mo..View full abstract
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Awarded by National Health and Medical Research Council of Australia
Awarded by Australian Research Council
This research was supported by National Health and Medical Research Council of Australia Project grants #1099016 (JF and JB) and #1006453 (JB), and Australian Research Council grant #DP130101596 (JB).