Journal article
An in vitro assay to measure antibody-mediated inhibition of P. berghei sporozoite invasion against P. falciparum antigens
A Rodríguez-Galán, AM Salman, G Bowyer, KA Collins, RJ Longley, F Brod, M Ulaszewska, KJ Ewer, CJ Janse, SM Khan, JC Hafalla, AVS Hill, AJ Spencer
Scientific Reports | NATURE PORTFOLIO | Published : 2017
Abstract
A large research effort is currently underway to find an effective and affordable malaria vaccine. Tools that enable the rapid evaluation of protective immune responses are essential to vaccine development as they can provide selection criteria to rank order vaccine candidates. In this study we have revisited the Inhibition of Sporozoite Invasion (ISI) assay to assess the ability of antibodies to inhibit sporozoite infection of hepatocytes. By using GFP expressing sporozoites of the rodent parasite P. berghei we are able to robustly quantify parasite infection of hepatocyte cell lines by flow cytometry. In conjunction with recently produced transgenic P. berghei parasites that express P. fal..
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Awarded by “la Caixa” Foundation
Funding Acknowledgements
The authors wish to thank V. Clark and H. Gray for their animal husbandry; A. Fyfe and S. Biswas for the production of mosquitoes; A. Worth for assistance with flow cytometry; and J. Furze for general laboratory assistance. This work has been funded by grants from the Wellcome Trust (095540/Z/11/Z and 104750/Z/14/Z) with personal support for AR funded by Fundacion "la Caixa", AS funded by BioMalpar and RJL funded in part by the Rhodes Trust. AVSH is a Wellcome Trust and NIHR Senior Investigator. The funding bodies did not have a role in the study design, data collection and analysis, decision to publish or preparation of the manuscript.