Fluorescent in situ identification of human marker chromosomes using flow sorting and Alu element-mediated PCR.
RF Suijkerbuijk, D Matthopoulos, L Kearney, S Monard, S Dhut, FE Cotter, J Herbergs, AG van Kessel, BD Young
Genomics | Published : 1992
A novel approach to the identification of human chromosomes has been developed. Chromosomal in situ hybridization (or "chromosome painting") has been performed using Alu element-mediated PCR products from small quantities (250-500) of flow-sorted normal and abnormal chromosomes. Chromosome paints for various normal chromosomes, including 5, 6, 7, 14, 18, 19, 21, and 22, were generated and shown to be effective in the identification of the appropriate chromosomes. In addition, certain abnormal chromosomes, including a mental retardation-associated deletion chromosome 11 (q22-q23), the products of the constitutional translocation t(11;22), and the CML-associated t(9;22), were used to generate ..View full abstract