Journal article

Improved single-chain transactivators of the Tet-on gene expression system

Xue Zhou, Jori Symons, Rieuwert Hoppes, Christel Krueger, Christian Berens, Wolfgang Hillen, Ben Berkhout, Atze T Das

BMC BIOTECHNOLOGY | BIOMED CENTRAL LTD | Published : 2007

Abstract

BACKGROUND: The Tet-Off (tTA) and Tet-On (rtTA) regulatory systems are widely applied to control gene expression in eukaryotes. Both systems are based on the Tet repressor (TetR) from transposon Tn10, a dimeric DNA-binding protein that binds to specific operator sequences (tetO). To allow the independent regulation of multiple genes, novel Tet systems are being developed that respond to different effectors and bind to different tetO sites. To prevent heterodimerization when multiple Tet systems are expressed in the same cell, single-chain variants of the transactivators have been constructed. Unfortunately, the activity of the single-chain rtTA (sc-rtTA) is reduced when compared with the reg..

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University of Melbourne Researchers