Identification of Indonesian Glade 2.1 highly pathogenic influenza A(H5N1) viruses with N294S and S246N neuraminidase substitutions which further reduce oseltamivir susceptibility
Jennifer L McKimm-Breschkin, Susan Barrett, Frank YK Wong, undefined Pudjiatmoko, Muhammad Azhar, Paul Selleck, Kelly R Davies, Nining Hartaningsih, James McGrane
Antiviral Research | ELSEVIER SCIENCE BV | Published : 2018
We have tested the in vitro susceptibility to the neuraminidase (NA) inhibitors of 96 highly pathogenic clade 2.1 A(H5N1) viruses from Indonesia, isolated between 2008 and 2011. HPAI virus samples obtained through the Influenza Virus Monitoring (IVM) surveillance program in Indonesia were tested for susceptibility to oseltamivir and zanamivir. The NAs of four viruses were identified as extreme outliers to oseltamivir, based on statistical analysis by box plots, with IC50 values ranging from 46 to 62 nM. The NAs of two of these viruses from Sumatra and Aceh, had an N294S substitution, while one virus from Sulawesi had an S246N NA substitution. The NAs of all four viruses showed a specific los..View full abstract
Awarded by US Agency for International Development (USAID)
We would like to thank the veterinary and diagnostic scientists of the regional Disease Investigation Centers (DIC) in Indonesia, and the Diagnosis, Surveillance and Response group of the CSIRO Australian Animal Health Laboratory (AAHL) for collection of samples, diagnosis and culture of viruses and DNA sequencing. The support and encouragement of the Director of Animal Health, Directorate General of Livestock and Animal Health Services, Ministry of Agriculture, Indonesia is gratefully acknowledged. This work was supported and partially funded by the US Agency for International Development (USAID) under the OSRO/INS/703/USA project "Monitoring AI virus variants in Indonesia poultry and defining an effective and sustainable vaccination strategy". The AAHL Facility is supported by the Australian Government Department of Agriculture and Water Resources and the National Collaborative Research Infrastructure Strategy (NCRIS).