Toward Worldwide Hepcidin Assay Harmonization: Identification of a Commutable Secondary Reference Material

Lisa N van der Vorm; Jan CM Hendriks; Coby M Laarakkers; Siem Klaver; Andrew E Armitage; Alison Bamberg; Anneke J Geurts-Moespot; Domenico Girelli; Matthias Herkert; Outi Itkonen; Robert J Konrad; Naohisa Tomosugi; Mark Westerman; Sukhvinder S Bansal; Natascia Campostrini; Hal Drakesmith; Marianne Fillet; Gordana Olbina; Sant-Rayn Pasricha; Kelly R Pitts; John H Sloan; Franco Tagliaro; Cas W Weykamp; Dorine W Swinkels

Journal article

Toward Worldwide Hepcidin Assay Harmonization: Identification of a Commutable Secondary Reference Material

Lisa N van der Vorm, Jan CM Hendriks, Coby M Laarakkers, Siem Klaver, Andrew E Armitage, Alison Bamberg, Anneke J Geurts-Moespot, Domenico Girelli, Matthias Herkert, Outi Itkonen, Robert J Konrad, Naohisa Tomosugi, Mark Westerman, Sukhvinder S Bansal, Natascia Campostrini, Hal Drakesmith, Marianne Fillet, Gordana Olbina, Sant-Rayn Pasricha, Kelly R Pitts Show all

CLINICAL CHEMISTRY | AMER ASSOC CLINICAL CHEMISTRY | Published : 2016

DOI: 10.1373/clinchem.2016.256768

Abstract

BACKGROUND: Absolute plasma hepcidin concentrations measured by various procedures differ substantially, complicating interpretation of results and rendering reference intervals method dependent. We investigated the degree of equivalence achievable by harmonization and the identification of a commutable secondary reference material to accomplish this goal. METHODS: We applied technical procedures to achieve harmonization developed by the Consortium for Harmonization of Clinical Laboratory Results. Eleven plasma hepcidin measurement procedures (5 mass spectrometry based and 6 immunochemical based) quantified native individual plasma samples (n = 32) and native plasma pools (n = 8) to assess a..

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