Journal article
MYR1-dependent effectors are the major drivers of a host cell’s early response to Toxoplasma, including counteracting MYR1-independent effects
A Naor, MW Panas, N Marino, MJ Coffey, CJ Tonkin, JC Boothroyd
Mbio | AMER SOC MICROBIOLOGY | Published : 2018
Abstract
The obligate intracellular parasite Toxoplasma gondii controls its host cell from within the parasitophorous vacuole (PV) by using a number of diverse effector proteins, a subset of which require the aspartyl protease 5 enzyme (ASP5) and/or the recently discovered MYR1 protein to cross the PV membrane. To examine the impact these effectors have in the context of the entirety of the host response to Toxoplasma, we used RNA-Seq to analyze the transcriptome expression profiles of human foreskin fibroblasts infected with wild-type RH (RH-WT), RHΔmyr1, and RHΔasp5 tachyzoites. Interestingly, the majority of the differentially regulated genes responding to Toxoplasma infection are MYR1 dependent. ..
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Awarded by National Institutes of Health
Funding Acknowledgements
A.N. was funded by the Human Frontier Science Program (LT000404/2014-L), J.B. was funded by NIH RO1 (AI021423) and NIH R21 (AI112962), and N.D.M. was funded by NIH F31-AI120649. C.J.T. and M.J.C. were supported by the David Winston Turner Foundation. C.J.T. would like to acknowledge the support of an ARC Future fellowship (FT120100164). C.J.T. and M.J.C. are also grateful for institutional support from the Victorian State Government Operational Infrastructure Support and the Australian Government NHMRC IRIISS.