Sequencing and Analysis of JC Virus DNA From Natalizumab-Treated PML Patients
Carl E Reid, Huo Li, Gargi Sur, Paul Carmillo, Steven Bushnell, Rich Tizard, Michele McAuliffe, Christopher Tonkin, Kenneth Simon, Susan Goelz, Paola Cinque, Leonid Gorelik, John P Carulli
The Journal of Infectious Diseases | OXFORD UNIV PRESS INC | Published : 2011
BACKGROUND: Progressive multifocal leukoencephalopathy (PML) in natalizumab-treated MS patients is linked to JC virus (JCV) infection. JCV sequence variation and rearrangements influence viral pathogenicity and tropism. To better understand PML development, we analyzed viral DNA sequences in blood, CSF and/or urine of natalizumab-treated PML patients. METHODS: Using biofluid samples from 17 natalizumab-treated PML patients, we sequenced multiple isolates of the JCV noncoding control region (NCCR), VP1 capsid coding region, and the entire 5 kb viral genome. RESULTS: Analysis of JCV from multiple biofluids revealed that individuals were infected with a single genotype. Across our patient cohor..View full abstract
This study was funded by Biogen Idec Inc. Funding to pay the Open Access publication charges for this paper was provided by Biogen Idec Inc.